EasyFig was recently added to our Galaxy tools to aid in comparing related genomes. Taking GenBank/EMBL files as inputs, users are able to visually compare anywhere from one to dozens of related genomes. As seen below, the mapping allows for rapid identification of variations between the genomes being evaluated. Parameters can be set to adjust minimum BLAST hit length, e-value and identity plotted to reduce image clutter and detect only the most homologous regions. BLAST results are run within the program, eliminating the need to run multiple jobs in order to visualize the data. Data is generated either as an HTML file or SVG file, an editable vector graphic great for preparing paper figures.
This tool wrapped by Anthony Criscione is in Galaxy under:
CPT: OTHER– EasyFig
Happy data crunching!
Please email the support team if you have any questions!