Phage Protocols

• Basic microbiology techniques
• Enrichment of environmental samples for phage
• Preparing a soft agar lawn of bacteria
• Making a phage pickate
• Streaking out phage for subculture
• Making a plate lysate
• Titering phage stocks
• Preparation of phage for transmission electron microscopy (TEM)
• Phage DNA extraction by phenol:chloroform method
• Phage purification by cesium chloride gradient