Phage Protocols
Commonly used wet-lab protocols
- Basic microbiology techniques
- Enrichment of environmental samples for phage
- Preparing a soft agar lawn of bacteria
- Making a phage pickate
- Streaking out phage for subculture
- Making a plate lysate
- Titering phage stocks
- Preparation of phage for transmission electron microscopy (TEM)
- Phage DNA extraction by phenol:chloroform method
- Phage purification by cesium chloride gradient
Comprehensive protocols on “Phage DNA Extraction, Genome Assembly, and Genome Closure” as part of a book (Chapter 8 in Methods Mol Biol, 2024:2738:125-144)
